As conclusion, from 48-h post fermented red oncom, a protease producing bacterial strain molecularly identified as Bacillus amyloliquefaciens strain IROD2. Based on the results of the molecular identification, the isolate was identified as Bacillus amyloliquefaciens strain IROD2 (IROD2 = Indonesia Red Oncom Day2). The result of the 16S rRNA gene sequence analysis showed that the proteolytic bacterial isolate obtained in this study had a 98% homology level with 16S ribosomal RNA isolate of Bacillus amyloliquefaciens strain A1142 (Genbank access code: KTT722836.1). From the isolation process a bacterial isolate that has proteolytic by the ability to produce a clear zone of 82.00 on plate. Identification process of the isolate was done through amplification of 16S rRNA gene using PCR, sequencing and analysis of gene sequences using BLAST program. Selection of protease enzyme obtained by bacterial isolate was done on Milk Skim Agar medium. enzim protease diisolasi dari bakteri Bacillus subtilis B1 dapat membantu hidrolisis protein pada susu kambing Peranakan Etawa. Dekomposisi protein oleh mikroorganisme lebih kompleks daripada pemecahan. Semua bakteri mempunyai enzim protease di dalam sel, tetapi tidak semua mempunyai enzim protease ekstraseluler.

The initial isolation and purification process of the colony was carried out using Nutrient Agar medium. Bakteri proteolitik adalah bakteri yang memproduksi enzim protease ekstraseluler, yaitu enzim pemecah protein yang diproduksi di dalam sel kemudian dilepaskan keluar dari sel. The PDF file you selected should load here if your Web browser has a PDF reader. This study aimed to isolate a proteolytic bacterium found on 48-h post-fermented oncom and molecular identification method. AKTIVITAS ENZIM AMILASE, LIPASE, DAN PROTEASE DARI LARVA Hermetia. Proteolytic bacteria are the bacteria capable of producing extracellular protease enzymes, namely protein-breaking enzymes that are widely used in many industrial fields.

Enzim proteolitik pdf